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ter119 microbeads  (Miltenyi Biotec)


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    Miltenyi Biotec ter119 microbeads
    a , Sanger sequencing results showing the Hba-x gene promoter sequence in the ZPE mouse model. Deletions of BCL11A and LRF motifs are indicated. b , Percentage of Hba-x mRNA to total β-like globin in E14.5 fetal liver cells and <t>Ter119</t> + bone marrow cells from WT and ZPE homozygotes ( n = 4 ZPE homozygous E14.5 embryos, n = 3 biological replicates for other groups). Data are presented as mean ± s.d. Bone marrow cells were isolated from male mice aged 13–15 weeks. c , ATAC–seq across the α-globin locus (mm10, chr11: 32,185,000–32,329,000) in fetal definitive erythroblasts from E12.5 WT and BHFS mouse embryos. The Hba-x gene is highlighted in green, the -- SEA deletion is highlighted in blue. The α-globin enhancers (R1–R4, Rm) are indicated. d , α-like globin mRNA expression in the WT, heterozygous and homozygous -- SEA deletion mouse models ( n = 5, 6 and 4 biological replicates, respectively). Data are presented as mean ± s.d. P values are shown; statistical significance was assessed using two-way ANOVA with Dunnett correction for multiple comparisons. e , Percentage of Hba-x mRNA to total β-like globin in peripheral blood from WT, BHFS and BHFS + ZPE E17.5 embryos ( n = 3, 3 and 2 biological replicates, respectively). Data are presented as mean values. f , Isoelectric focusing of extracts from E17.5 peripheral blood of WT, BHFS and BHFS + ZPE embryos. Extracts from E14.5 peripheral blood (lane 1) are used as mouse hemoglobin markers and positive controls for Hb Portland 2 (ζ 2 β 2 ). Experiments were independently repeated thrice with similar results. g , Representative microscopy of WT and BHFS mouse embryos at E15.5–E17.5, white triangles indicate signs of edema. Scale bar = 2 mm. Next to the embryos, representative cytospins of peripheral blood from E17.5 embryos stained using a modified Wright’s procedure. Scale bar = 10 μm. Right, flow cytometry analysis of erythroid enucleation of peripheral blood from E17.5 embryos. Enucleated cells are identified as Hoechst 33342-negative cells. FSC, forward scatter. M, million. Similar results were obtained from at least two independent embryos.
    Ter119 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 2195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ter+119/Ter-119+Antibody%2C+anti-mouse/pmc13649559-286-16-18
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    Images

    1) Product Images from "Reactivation of the embryonic ζ-globin gene ameliorates severe forms of α-thalassemia"

    Article Title: Reactivation of the embryonic ζ-globin gene ameliorates severe forms of α-thalassemia

    Journal: Nature Genetics

    doi: 10.1038/s41588-026-02770-0

    a , Sanger sequencing results showing the Hba-x gene promoter sequence in the ZPE mouse model. Deletions of BCL11A and LRF motifs are indicated. b , Percentage of Hba-x mRNA to total β-like globin in E14.5 fetal liver cells and Ter119 + bone marrow cells from WT and ZPE homozygotes ( n = 4 ZPE homozygous E14.5 embryos, n = 3 biological replicates for other groups). Data are presented as mean ± s.d. Bone marrow cells were isolated from male mice aged 13–15 weeks. c , ATAC–seq across the α-globin locus (mm10, chr11: 32,185,000–32,329,000) in fetal definitive erythroblasts from E12.5 WT and BHFS mouse embryos. The Hba-x gene is highlighted in green, the -- SEA deletion is highlighted in blue. The α-globin enhancers (R1–R4, Rm) are indicated. d , α-like globin mRNA expression in the WT, heterozygous and homozygous -- SEA deletion mouse models ( n = 5, 6 and 4 biological replicates, respectively). Data are presented as mean ± s.d. P values are shown; statistical significance was assessed using two-way ANOVA with Dunnett correction for multiple comparisons. e , Percentage of Hba-x mRNA to total β-like globin in peripheral blood from WT, BHFS and BHFS + ZPE E17.5 embryos ( n = 3, 3 and 2 biological replicates, respectively). Data are presented as mean values. f , Isoelectric focusing of extracts from E17.5 peripheral blood of WT, BHFS and BHFS + ZPE embryos. Extracts from E14.5 peripheral blood (lane 1) are used as mouse hemoglobin markers and positive controls for Hb Portland 2 (ζ 2 β 2 ). Experiments were independently repeated thrice with similar results. g , Representative microscopy of WT and BHFS mouse embryos at E15.5–E17.5, white triangles indicate signs of edema. Scale bar = 2 mm. Next to the embryos, representative cytospins of peripheral blood from E17.5 embryos stained using a modified Wright’s procedure. Scale bar = 10 μm. Right, flow cytometry analysis of erythroid enucleation of peripheral blood from E17.5 embryos. Enucleated cells are identified as Hoechst 33342-negative cells. FSC, forward scatter. M, million. Similar results were obtained from at least two independent embryos.
    Figure Legend Snippet: a , Sanger sequencing results showing the Hba-x gene promoter sequence in the ZPE mouse model. Deletions of BCL11A and LRF motifs are indicated. b , Percentage of Hba-x mRNA to total β-like globin in E14.5 fetal liver cells and Ter119 + bone marrow cells from WT and ZPE homozygotes ( n = 4 ZPE homozygous E14.5 embryos, n = 3 biological replicates for other groups). Data are presented as mean ± s.d. Bone marrow cells were isolated from male mice aged 13–15 weeks. c , ATAC–seq across the α-globin locus (mm10, chr11: 32,185,000–32,329,000) in fetal definitive erythroblasts from E12.5 WT and BHFS mouse embryos. The Hba-x gene is highlighted in green, the -- SEA deletion is highlighted in blue. The α-globin enhancers (R1–R4, Rm) are indicated. d , α-like globin mRNA expression in the WT, heterozygous and homozygous -- SEA deletion mouse models ( n = 5, 6 and 4 biological replicates, respectively). Data are presented as mean ± s.d. P values are shown; statistical significance was assessed using two-way ANOVA with Dunnett correction for multiple comparisons. e , Percentage of Hba-x mRNA to total β-like globin in peripheral blood from WT, BHFS and BHFS + ZPE E17.5 embryos ( n = 3, 3 and 2 biological replicates, respectively). Data are presented as mean values. f , Isoelectric focusing of extracts from E17.5 peripheral blood of WT, BHFS and BHFS + ZPE embryos. Extracts from E14.5 peripheral blood (lane 1) are used as mouse hemoglobin markers and positive controls for Hb Portland 2 (ζ 2 β 2 ). Experiments were independently repeated thrice with similar results. g , Representative microscopy of WT and BHFS mouse embryos at E15.5–E17.5, white triangles indicate signs of edema. Scale bar = 2 mm. Next to the embryos, representative cytospins of peripheral blood from E17.5 embryos stained using a modified Wright’s procedure. Scale bar = 10 μm. Right, flow cytometry analysis of erythroid enucleation of peripheral blood from E17.5 embryos. Enucleated cells are identified as Hoechst 33342-negative cells. FSC, forward scatter. M, million. Similar results were obtained from at least two independent embryos.

    Techniques Used: DNA Sequencing, Sequencing, Isolation, Expressing, Microscopy, Staining, Modification, Flow Cytometry, Analysis

    Related Articles

    Staining:

    Article Title: The TET-dependent DNA demethylation pathway is the driving force of hematopoiesis
    Article Snippet: Data analysis was performed with FlowJo software. .. Bone marrow and spleen cell suspensions were stained using the following antibodies: anti-Gr-1 (130-112-311, Miltenyi Biotec), anti-CD11b (130-113-797), anti-CD117 (130-111-694, Miltenyi Biotec), anti-CD71 (130-120-809, Miltenyi Biotec), anti-Ter-119 (130-117-538, Miltenyi Biotec), anti-CD8 (130-111-710, Miltenyi Biotec), anti-CD4 (130-116-509, Miltenyi Biotec), anti-CD19 (130-112-035, Miltenyi Biotec), and anti-CD45R (B220) (130-110-845, Miltenyi Biotec). ..

    Incubation:

    Article Title: SOXC are critical regulators of adult bone mass
    Article Snippet: .. Briefly, cells were incubated with biotinylated antibodies against hematopoietic markers, including the mouse lineage cell depletion kit (130-090-858, Miltenyi Biotec), and anti-TER-119 (13-5921-85, Thermo Fisher Scientific), anti-CD71 (130-109-572, Miltenyi Biotec), and anti-CD3e (130-093-179, Miltenyi Biotec) antibodies. .. Cells were then incubated with anti-biotin magnetic microbeads (130-090-485, Miltenyi Biotec) and sorted through LS columns (130-042-401, Miltenyi Biotec) containing ferromagnetic spheres and mounted on a MACS separator (130-090-976, Miltenyi Biotec).

    Article Title: Epi-microRNA mediated metabolic reprogramming counteracts hypoxia to preserve affinity maturation.
    Article Snippet: .. For HELspecific GC responses, splenic cells from donor mice were incubated with biotinylated antibodies: anti-CD43, anti-CD4 and anti-Ter-119, washed and incubated with anti-biotin microbeads (Miltenyi Biotec). .. B cells were enriched by biotin negative selection using MACS LS Columns (Miltenyi Biotec).

    Article Title: SOXC are critical regulators of adult bone mass.
    Article Snippet: .. Briefly, cells were incubated with biotinylated antibodies against hematopoietic markers, including the mouse lineage cell depletion kit (130-090-858, Miltenyi Biotec), and anti-TER-119 (13- 5921-85, Thermo Fisher Scientific), anti-CD71 (130-109-572, Miltenyi Biotec), and anti-CD3e (130-093-179, Miltenyi Biotec) antibodies. .. Cells were then incubated with anti-biotin magnetic microbeads (130-090- 485, Miltenyi Biotec) and sorted through LS columns (130-042-401, Miltenyi Biotec) containing ferromagnetic spheres and mounted on a MACS separator (130-090-976, Miltenyi Biotec).

    Article Title: Adipose Triglyceride Lipase Deficiency Attenuates In Vitro Thrombus Formation without Affecting Platelet Activation and Bleeding In Vivo.
    Article Snippet: The upper layers containing platelets were pooled and mixed with 1/25 volume of ACD and 1/100 volume of apyrase (50 U/mL; Sigma-Aldrich) to inhibit preactivation. .. To purify platelets, cells were incubated with anti-Ter-119 and anti-CD45 beads (Miltenyi Biotec, Bergisch Gladbach, Germany) to remove residual red blood cells and leukocytes, respectively. ..

    Article Title: Epi-microRNA mediated metabolic reprogramming counteracts hypoxia to preserve affinity maturation
    Article Snippet: .. For HEL-specific GC responses, splenic cells from donor mice were incubated with biotinylated antibodies: anti-CD43, anti-CD4 and anti-Ter-119, washed and incubated with anti-biotin microbeads (Miltenyi Biotec). .. B cells were enriched by biotin negative selection using MACS LS Columns (Miltenyi Biotec).

    Article Title: Monoglyceride Lipase Deficiency Is Associated with Altered Thrombogenesis in Mice.
    Article Snippet: .. To remove residual red blood cells and leukocytes for pure platelet preparations, cells were incubated with anti-Ter-119 and anti-CD45 beads (Miltenyi Biotec, Bergisch Gladbach, Germany), respectively. ..

    Article Title: Monoglyceride Lipase Deficiency Is Associated with Altered Thrombogenesis in Mice
    Article Snippet: .. To remove residual red blood cells and leukocytes for pure platelet preparations, cells were incubated with anti-Ter-119 and anti-CD45 beads (Miltenyi Biotec, Bergisch Gladbach, Germany), respectively. ..



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    a , Sanger sequencing results showing the Hba-x gene promoter sequence in the ZPE mouse model. Deletions of BCL11A and LRF motifs are indicated. b , Percentage of Hba-x mRNA to total β-like globin in E14.5 fetal liver cells and <t>Ter119</t> + bone marrow cells from WT and ZPE homozygotes ( n = 4 ZPE homozygous E14.5 embryos, n = 3 biological replicates for other groups). Data are presented as mean ± s.d. Bone marrow cells were isolated from male mice aged 13–15 weeks. c , ATAC–seq across the α-globin locus (mm10, chr11: 32,185,000–32,329,000) in fetal definitive erythroblasts from E12.5 WT and BHFS mouse embryos. The Hba-x gene is highlighted in green, the -- SEA deletion is highlighted in blue. The α-globin enhancers (R1–R4, Rm) are indicated. d , α-like globin mRNA expression in the WT, heterozygous and homozygous -- SEA deletion mouse models ( n = 5, 6 and 4 biological replicates, respectively). Data are presented as mean ± s.d. P values are shown; statistical significance was assessed using two-way ANOVA with Dunnett correction for multiple comparisons. e , Percentage of Hba-x mRNA to total β-like globin in peripheral blood from WT, BHFS and BHFS + ZPE E17.5 embryos ( n = 3, 3 and 2 biological replicates, respectively). Data are presented as mean values. f , Isoelectric focusing of extracts from E17.5 peripheral blood of WT, BHFS and BHFS + ZPE embryos. Extracts from E14.5 peripheral blood (lane 1) are used as mouse hemoglobin markers and positive controls for Hb Portland 2 (ζ 2 β 2 ). Experiments were independently repeated thrice with similar results. g , Representative microscopy of WT and BHFS mouse embryos at E15.5–E17.5, white triangles indicate signs of edema. Scale bar = 2 mm. Next to the embryos, representative cytospins of peripheral blood from E17.5 embryos stained using a modified Wright’s procedure. Scale bar = 10 μm. Right, flow cytometry analysis of erythroid enucleation of peripheral blood from E17.5 embryos. Enucleated cells are identified as Hoechst 33342-negative cells. FSC, forward scatter. M, million. Similar results were obtained from at least two independent embryos.
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    a , Sanger sequencing results showing the Hba-x gene promoter sequence in the ZPE mouse model. Deletions of BCL11A and LRF motifs are indicated. b , Percentage of Hba-x mRNA to total β-like globin in E14.5 fetal liver cells and Ter119 + bone marrow cells from WT and ZPE homozygotes ( n = 4 ZPE homozygous E14.5 embryos, n = 3 biological replicates for other groups). Data are presented as mean ± s.d. Bone marrow cells were isolated from male mice aged 13–15 weeks. c , ATAC–seq across the α-globin locus (mm10, chr11: 32,185,000–32,329,000) in fetal definitive erythroblasts from E12.5 WT and BHFS mouse embryos. The Hba-x gene is highlighted in green, the -- SEA deletion is highlighted in blue. The α-globin enhancers (R1–R4, Rm) are indicated. d , α-like globin mRNA expression in the WT, heterozygous and homozygous -- SEA deletion mouse models ( n = 5, 6 and 4 biological replicates, respectively). Data are presented as mean ± s.d. P values are shown; statistical significance was assessed using two-way ANOVA with Dunnett correction for multiple comparisons. e , Percentage of Hba-x mRNA to total β-like globin in peripheral blood from WT, BHFS and BHFS + ZPE E17.5 embryos ( n = 3, 3 and 2 biological replicates, respectively). Data are presented as mean values. f , Isoelectric focusing of extracts from E17.5 peripheral blood of WT, BHFS and BHFS + ZPE embryos. Extracts from E14.5 peripheral blood (lane 1) are used as mouse hemoglobin markers and positive controls for Hb Portland 2 (ζ 2 β 2 ). Experiments were independently repeated thrice with similar results. g , Representative microscopy of WT and BHFS mouse embryos at E15.5–E17.5, white triangles indicate signs of edema. Scale bar = 2 mm. Next to the embryos, representative cytospins of peripheral blood from E17.5 embryos stained using a modified Wright’s procedure. Scale bar = 10 μm. Right, flow cytometry analysis of erythroid enucleation of peripheral blood from E17.5 embryos. Enucleated cells are identified as Hoechst 33342-negative cells. FSC, forward scatter. M, million. Similar results were obtained from at least two independent embryos.

    Journal: Nature Genetics

    Article Title: Reactivation of the embryonic ζ-globin gene ameliorates severe forms of α-thalassemia

    doi: 10.1038/s41588-026-02770-0

    Figure Lengend Snippet: a , Sanger sequencing results showing the Hba-x gene promoter sequence in the ZPE mouse model. Deletions of BCL11A and LRF motifs are indicated. b , Percentage of Hba-x mRNA to total β-like globin in E14.5 fetal liver cells and Ter119 + bone marrow cells from WT and ZPE homozygotes ( n = 4 ZPE homozygous E14.5 embryos, n = 3 biological replicates for other groups). Data are presented as mean ± s.d. Bone marrow cells were isolated from male mice aged 13–15 weeks. c , ATAC–seq across the α-globin locus (mm10, chr11: 32,185,000–32,329,000) in fetal definitive erythroblasts from E12.5 WT and BHFS mouse embryos. The Hba-x gene is highlighted in green, the -- SEA deletion is highlighted in blue. The α-globin enhancers (R1–R4, Rm) are indicated. d , α-like globin mRNA expression in the WT, heterozygous and homozygous -- SEA deletion mouse models ( n = 5, 6 and 4 biological replicates, respectively). Data are presented as mean ± s.d. P values are shown; statistical significance was assessed using two-way ANOVA with Dunnett correction for multiple comparisons. e , Percentage of Hba-x mRNA to total β-like globin in peripheral blood from WT, BHFS and BHFS + ZPE E17.5 embryos ( n = 3, 3 and 2 biological replicates, respectively). Data are presented as mean values. f , Isoelectric focusing of extracts from E17.5 peripheral blood of WT, BHFS and BHFS + ZPE embryos. Extracts from E14.5 peripheral blood (lane 1) are used as mouse hemoglobin markers and positive controls for Hb Portland 2 (ζ 2 β 2 ). Experiments were independently repeated thrice with similar results. g , Representative microscopy of WT and BHFS mouse embryos at E15.5–E17.5, white triangles indicate signs of edema. Scale bar = 2 mm. Next to the embryos, representative cytospins of peripheral blood from E17.5 embryos stained using a modified Wright’s procedure. Scale bar = 10 μm. Right, flow cytometry analysis of erythroid enucleation of peripheral blood from E17.5 embryos. Enucleated cells are identified as Hoechst 33342-negative cells. FSC, forward scatter. M, million. Similar results were obtained from at least two independent embryos.

    Article Snippet: Adult definitive erythroid cells were obtained from mouse bone marrow by positive selection for Ter119 using Ter119 microbeads (Miltenyi Biotec).

    Techniques: DNA Sequencing, Sequencing, Isolation, Expressing, Microscopy, Staining, Modification, Flow Cytometry, Analysis